Trypan blue

What is Trypan blue

Trypan blue is an acid di-azo group dye approved for use as an aid in ophthalmic surgery by selective tissue staining.

Trypan blue is available in 2 concentrations, a 0.06% solution indicated for staining of the anterior lens capsule and a 0.15% solution indicated for staining of the retinal membranes

Brand Name



  • ophthalmic surgery

For use as a visualization aid during ophthalmic surgery

Side Effects

  • no side effects

In a clinical study of trypan blue in 6 eyes of 6 different participants, no adverse reactions were observed up to 1 year after surgery.

However, discoloration of certain intraocular lenses and inadvertent staining of the posterior lens capsule and vitreous face have been noted during use of trypan blue in ophthalmic cataract removal surgery. Staining of the posterior lens capsule or vitreous face is generally self-limited, lasting up to 1 week.

Monitoring Parameters

  • laboratory monitoring not necessary


  • breast-feeding
  • pregnancy


There are no drug interactions associated with Trypan blue products.


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